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ATCC human lens epithelial cell line
Human Lens Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+lens+epithelial+cell+line+b+3/B-3/pmc12988590-56-38-49
Average 97 stars, based on 647 article reviews

human lens epithelial cell line - by Bioz Stars, 2026-10

97/100 stars

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Cell Culture:

Article Title: Collective Migration of Lens Epithelial Cell Induced by Differential Microscale Groove Patterns
Article Snippet: Then, the molds were immersed in high-purity ethanol and kept in a clean bench with UV light on for at least 2 h. The sterilized PDMS molds were coated with fibronectin (Bovine, Sigma-Aldrich, St. Louis, MO, USA) before cell seeding. .. Human lens epithelial cell line B-3 (ATCC, Manassas, WV, USA) was cultured in minimum essential medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20% fetal bovine serum (FBS, Welgene, Gyeongsan, Korea) and 1% Antibiotic-Antimycotic (Gibco, Thermo Fisher Scientific, Waltham, Massachusetts, USA) at 37 °C, 5% CO 2 , and 90% humidity. ..

Article Title: SIRT1 Prevents Lens Epithelial Cell Senescence During Age‐Related Cataract via Regulating p66Shc
Article Snippet: .. The human lens epithelial cell line B‐3 (HLE‐B3) was purchased from the American Type Culture Collection (ATCC Cat# CRL‐11421, RRID: CVCL_6367) and cultured in a humidified incubator at 37°C with 5% CO 2 in modified Eagle's medium supplemented with 15% fetal bovine serum and 1% penicillin–streptomycin. ..

Modification:

Image Search Results

FIGURE 1. lncRNA HOTAIR expression and localization in the PCO model. (A, B) mRNA levels of lncRNA HOTAIR, vimentin, N-cadherin, E-cadherin, and ZEB1 in LECs of the NS group and 5 days post-surgery were determined using qRT-PCR analysis. (C, D) Protein levels of EMT markers in LECs of the NS group and 5 days post-surgery were determined using western blotting analysis. (E, F) lncRNA HOTAIR, vimentin, N-cadherin, E-cadherin, and ZEB1 mRNA levels after a 48-hour treatment of LECs with TGF-β2 (10 ng/mL). (G, H) Protein levels of EMT markers in TGF-β2 (10 ng/mL, 48 hours)–induced LECs. (I) Nuclear localization of lncRNA HOTAIR in HLE-B3 cells by RNA-FISH assay. Scale bar: 20 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 1. lncRNA HOTAIR expression and localization in the PCO model. (A, B) mRNA levels of lncRNA HOTAIR, vimentin, N-cadherin, E-cadherin, and ZEB1 in LECs of the NS group and 5 days post-surgery were determined using qRT-PCR analysis. (C, D) Protein levels of EMT markers in LECs of the NS group and 5 days post-surgery were determined using western blotting analysis. (E, F) lncRNA HOTAIR, vimentin, N-cadherin, E-cadherin, and ZEB1 mRNA levels after a 48-hour treatment of LECs with TGF-β2 (10 ng/mL). (G, H) Protein levels of EMT markers in TGF-β2 (10 ng/mL, 48 hours)–induced LECs. (I) Nuclear localization of lncRNA HOTAIR in HLE-B3 cells by RNA-FISH assay. Scale bar: 20 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

FIGURE 2. LncRNA HOTAIR overexpression promotes EMT, cell viability, proliferation, and migration of TGF-l –treated LECs. (A, B) After transfection of the HLE-B3 cells with HOTAIR overexpression or NC lentivirus followed by treatment with 10 ng/mL TGF-β2 for 48 hours, western blotting indicated vimentin, N-cadherin, and E-cadherin protein levels. (C) The viability of HLE-B3 cells was examined using the CCK-8 assay. (D, E) The proliferation of HLE-B3 cells was examined using EdU analysis. Scale bar: 200 μm. (F, G) Transwell migration results expressed as the number of migrated cells. Scale bar: 200 μm. (H, I) Wound healing assay showed cell migration at 0 and 48 hours. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 2. LncRNA HOTAIR overexpression promotes EMT, cell viability, proliferation, and migration of TGF-l –treated LECs. (A, B) After transfection of the HLE-B3 cells with HOTAIR overexpression or NC lentivirus followed by treatment with 10 ng/mL TGF-β2 for 48 hours, western blotting indicated vimentin, N-cadherin, and E-cadherin protein levels. (C) The viability of HLE-B3 cells was examined using the CCK-8 assay. (D, E) The proliferation of HLE-B3 cells was examined using EdU analysis. Scale bar: 200 μm. (F, G) Transwell migration results expressed as the number of migrated cells. Scale bar: 200 μm. (H, I) Wound healing assay showed cell migration at 0 and 48 hours. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Over Expression, Migration, Transfection, Western Blot, CCK-8 Assay, Wound Healing Assay

FIGURE 3. LncRNA HOTAIR knockdown inhibits TGF-bi–induced EMT, cell viability, proliferation, and migration of LECs. (A, B) After transfection of the HLE-B3 cells with si-HOTAIR or si-NC followed by treatment with 10 ng/mL TGF-β2 for 48 hours, western blotting indicated EMT marker protein levels. (C) The viability of HLE-B3 cells was examined using the CCK-8 assay. (D, E) The proliferation of HLE-B3 cells was examined using EdU analysis. Scale bar: 200 μm. (F, G) Transwell migration results expressed as the number of migrated cells. Scale bar: 200 μm. (H, I) Wound healing assay showed cell migration at 0 and 48 hours. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 3. LncRNA HOTAIR knockdown inhibits TGF-bi–induced EMT, cell viability, proliferation, and migration of LECs. (A, B) After transfection of the HLE-B3 cells with si-HOTAIR or si-NC followed by treatment with 10 ng/mL TGF-β2 for 48 hours, western blotting indicated EMT marker protein levels. (C) The viability of HLE-B3 cells was examined using the CCK-8 assay. (D, E) The proliferation of HLE-B3 cells was examined using EdU analysis. Scale bar: 200 μm. (F, G) Transwell migration results expressed as the number of migrated cells. Scale bar: 200 μm. (H, I) Wound healing assay showed cell migration at 0 and 48 hours. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Knockdown, Migration, Transfection, Western Blot, Marker, CCK-8 Assay, Wound Healing Assay

FIGURE 4. Total m6A levels and expression and localization of WTAP, METTL3, and METTL14 in the PCO model. (A) RNA m6A quantification assay showed total m6A modification levels in LECs from the NS and 1, 2, 3, 4, and 5 days after cataract surgery. (B) Total m6A modification level in TGF-β2 (10 ng/mL)–treated LECs after 48 hours. (C–E) mRNA and protein levels of WTAP, METTL3, and METTL14 in LECs from the NS group and 5 days post-surgery determined using qRT-PCR and western blotting analysis. (F–H) mRNA and protein levels of WTAP, METTL3, and METTL14 in TGF-β2 (10 ng/mL)–treated LECs after 48 hours. (I) IF staining assay indicated nuclear localization of WTAP, METTL3, and METTL14 (green) in HLE-B3 cells, with nuclei stained with DAPI (blue). Scale bar: 40 μm. (J–L) IF staining assay indicated the expression levels of WTAP, METTL3, and METTL14 (green) in lens capsules of the NS group and 5 days post-surgery, with nuclei stained with DAPI (blue). Scale bar: 50 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 4. Total m6A levels and expression and localization of WTAP, METTL3, and METTL14 in the PCO model. (A) RNA m6A quantification assay showed total m6A modification levels in LECs from the NS and 1, 2, 3, 4, and 5 days after cataract surgery. (B) Total m6A modification level in TGF-β2 (10 ng/mL)–treated LECs after 48 hours. (C–E) mRNA and protein levels of WTAP, METTL3, and METTL14 in LECs from the NS group and 5 days post-surgery determined using qRT-PCR and western blotting analysis. (F–H) mRNA and protein levels of WTAP, METTL3, and METTL14 in TGF-β2 (10 ng/mL)–treated LECs after 48 hours. (I) IF staining assay indicated nuclear localization of WTAP, METTL3, and METTL14 (green) in HLE-B3 cells, with nuclei stained with DAPI (blue). Scale bar: 40 μm. (J–L) IF staining assay indicated the expression levels of WTAP, METTL3, and METTL14 (green) in lens capsules of the NS group and 5 days post-surgery, with nuclei stained with DAPI (blue). Scale bar: 50 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Expressing, Modification, Quantitative RT-PCR, Western Blot, Staining, Capsules

FIGURE 5. Interaction between lncRNA HOTAIR and WTAP protein and the effect of HOTAIR on the WTAP/METTL3/METTL14 complex and total m6A level in EMT of LECs. (A, B) RIP and qRT-PCR showed the interaction between HOTAIR and WTAP. (C, D) HOTAIR expression levels were examined using qRT-PCR in HLE-B3 cells with overexpression and knockdown of WTAP. (E, H) Total m6A level in HOTAIR overexpression and knockdown LECs after TGF-β2 treatment (10 ng/mL for 48 hours). (F, G, I, J) Western blotting indicated WTAP, METTL3, and METTL14 protein levels in HOTAIR overexpression and knockdown LECs after TGF-β2 treatment (10 ng/mL for 48 hours). (K) Dual RNA- FISH and IF indicated the colocalization of HOTAIR and WTAP in TGF-β2–induced HLE-B3 cells with low or overexpression of HOTAIR. Scale bar: 30 μm. (L) Co-IP and western blotting results showed the binding efficiency of WTAP with METTL3 and METTL14 in LECs transfected with si-NC or si- HOTAIR, and co-transfected with NC or WTAP overexpression lentivirus. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test and one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 5. Interaction between lncRNA HOTAIR and WTAP protein and the effect of HOTAIR on the WTAP/METTL3/METTL14 complex and total m6A level in EMT of LECs. (A, B) RIP and qRT-PCR showed the interaction between HOTAIR and WTAP. (C, D) HOTAIR expression levels were examined using qRT-PCR in HLE-B3 cells with overexpression and knockdown of WTAP. (E, H) Total m6A level in HOTAIR overexpression and knockdown LECs after TGF-β2 treatment (10 ng/mL for 48 hours). (F, G, I, J) Western blotting indicated WTAP, METTL3, and METTL14 protein levels in HOTAIR overexpression and knockdown LECs after TGF-β2 treatment (10 ng/mL for 48 hours). (K) Dual RNA- FISH and IF indicated the colocalization of HOTAIR and WTAP in TGF-β2–induced HLE-B3 cells with low or overexpression of HOTAIR. Scale bar: 30 μm. (L) Co-IP and western blotting results showed the binding efficiency of WTAP with METTL3 and METTL14 in LECs transfected with si-NC or si- HOTAIR, and co-transfected with NC or WTAP overexpression lentivirus. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test and one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Quantitative RT-PCR, Expressing, Over Expression, Knockdown, Western Blot, Co-Immunoprecipitation Assay, Binding Assay, Transfection

FIGURE 7. Identification of the lncRNA HOTAIR/WTAP complex targets via RNA-seq and MeRIP-seq. (A) DEGs identified by RNA-seq in NC and HOTAIR overexpression HLE-B3 cells with TGF-β2 treatment. (B) Heatmap showed consistency between NC and HOTAIR overexpression groups. (C) Predominant consensus motif GGAC. (D) The number of m6A peaks in NC and HOTAIR overexpression groups. (E) Density distribution of m6A peaks across mRNA transcripts. (F) Distribution of different m6A peaks in NC and HOTAIR overexpression groups. (G, H) GO and KEGG enrichment analysis of the genes of differential peaks.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 7. Identification of the lncRNA HOTAIR/WTAP complex targets via RNA-seq and MeRIP-seq. (A) DEGs identified by RNA-seq in NC and HOTAIR overexpression HLE-B3 cells with TGF-β2 treatment. (B) Heatmap showed consistency between NC and HOTAIR overexpression groups. (C) Predominant consensus motif GGAC. (D) The number of m6A peaks in NC and HOTAIR overexpression groups. (E) Density distribution of m6A peaks across mRNA transcripts. (F) Distribution of different m6A peaks in NC and HOTAIR overexpression groups. (G, H) GO and KEGG enrichment analysis of the genes of differential peaks.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: RNA Sequencing, Over Expression

FIGURE 8. THBS1 serves as the downstream marker of the HOTAIR/WTAP complex in LECs EMT. (A) m6A levels of lncRNA HOTAIR did not change in the control group or TGF-β2 treatment group. (B–F) RIP-qPCR revealed the relative m6A modification levels of five mRNA candidates (LZTS1, THBS1, FOSB, KLF6, and FZD5) in TGF-β2–induced HLE-B3 cells stably transfected with NC or HOTAIR overexpression lentivirus. (G) The levels of five mRNA candidates in TGF-β2–induced HLE-B3 cells stably transfected with NC or HOTAIR overexpression lentivirus were detected using qRT-PCR assay. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test and one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 8. THBS1 serves as the downstream marker of the HOTAIR/WTAP complex in LECs EMT. (A) m6A levels of lncRNA HOTAIR did not change in the control group or TGF-β2 treatment group. (B–F) RIP-qPCR revealed the relative m6A modification levels of five mRNA candidates (LZTS1, THBS1, FOSB, KLF6, and FZD5) in TGF-β2–induced HLE-B3 cells stably transfected with NC or HOTAIR overexpression lentivirus. (G) The levels of five mRNA candidates in TGF-β2–induced HLE-B3 cells stably transfected with NC or HOTAIR overexpression lentivirus were detected using qRT-PCR assay. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test and one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Marker, Control, Modification, Stable Transfection, Transfection, Over Expression, Quantitative RT-PCR

FIGURE 9. HOTAIR/WTAP complex enhances the expression of THBS1 in an m6A-dependent manner in the EMT of LECs. (A–F) Western blotting showed the expression levels of LZTS1, THBS1, FOSB, KLF6, and FZD5 in TGF-β2–induced HLE-B3 with stable NC or HOTAIR overexpression lentivirus transfection. (G, H) Western blotting showed THBS1 protein levels in TGF-β2–induced WTAP overexpression HLE- B3 cells. (I–K) After TGF-β2–induced HLE-B3 cells were transfected with si-NC or si-HOTAIR and then co-transfected with NC or WTAP overexpression lentivirus, RIP-qPCR showed the relative m6A modification levels of THBS1 mRNA. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 9. HOTAIR/WTAP complex enhances the expression of THBS1 in an m6A-dependent manner in the EMT of LECs. (A–F) Western blotting showed the expression levels of LZTS1, THBS1, FOSB, KLF6, and FZD5 in TGF-β2–induced HLE-B3 with stable NC or HOTAIR overexpression lentivirus transfection. (G, H) Western blotting showed THBS1 protein levels in TGF-β2–induced WTAP overexpression HLE- B3 cells. (I–K) After TGF-β2–induced HLE-B3 cells were transfected with si-NC or si-HOTAIR and then co-transfected with NC or WTAP overexpression lentivirus, RIP-qPCR showed the relative m6A modification levels of THBS1 mRNA. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Expressing, Western Blot, Over Expression, Transfection, Modification

FIGURE 11. HOTAIR/WTAP complex promotes EMT of LECs by enhancing THBS1 expression. (A–D) Western blotting analysis showed the expression levels of EMT markers after TGF-β2–induced HLE-B3 cells were transfected with NC or HOTAIR overexpression followed by co- transfection with si-NC or si-THBS1. (E) IF staining showed the vimentin level. Scale bar: 100 μm. (F, G) Transwell assay showed migrating cells in TGF-β2-induced HOTAIR overexpression HLE-B3 cells with THBS1 low expression. Scale bar: 200 μm. (H, I) Wound healing assay showed migrating cells in TGF-β2–induced HOTAIR overexpression HLE-B3 cells with THBS1 low expression. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way ANOVA); ns, not significant.

Journal: Investigative ophthalmology & visual science

Article Title: LncRNA HOTAIR Interaction With WTAP Promotes m6A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1.

doi: 10.1167/iovs.66.5.20

Figure Lengend Snippet: FIGURE 11. HOTAIR/WTAP complex promotes EMT of LECs by enhancing THBS1 expression. (A–D) Western blotting analysis showed the expression levels of EMT markers after TGF-β2–induced HLE-B3 cells were transfected with NC or HOTAIR overexpression followed by co- transfection with si-NC or si-THBS1. (E) IF staining showed the vimentin level. Scale bar: 100 μm. (F, G) Transwell assay showed migrating cells in TGF-β2-induced HOTAIR overexpression HLE-B3 cells with THBS1 low expression. Scale bar: 200 μm. (H, I) Wound healing assay showed migrating cells in TGF-β2–induced HOTAIR overexpression HLE-B3 cells with THBS1 low expression. Scale bar: 100 μm. Data are presented as mean ± SEM (n = 3). Only P < 0.05 was considered significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way ANOVA); ns, not significant.

Article Snippet: The human lens epithelial B3 cell line (HLE-B3) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) (Supplementary Fig. S1D).

Techniques: Expressing, Western Blot, Transfection, Over Expression, Cotransfection, Staining, Transwell Assay, Wound Healing Assay

Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated HLE-B3 cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Journal: BMC ophthalmology

Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

doi: 10.1186/s12886-024-03746-6

Figure Lengend Snippet: Fig. 2 The effects of apelin-13 on the cell viability of H2O2-treated HLE-B3 cells. (a) HLE-B3 cells were incubated with various concentrations of apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h. (b) HLE-B3 cells were incubated with various concentrations of H2O2 (50 µM, 100 µM, 200 µM, 400 µM) for 24 h. (c) HLE- B3 cells were preincubated with apelin-13 (0.01 µM, 0.1 µM, 1 µM) for 24 h before being treated with H2O2 (200 µM) for 24 h. Cell viability was evaluated using CCK8 assay. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

Techniques: Incubation, CCK-8 Assay, Comparison, Control

Fig. 3 Apelin-13 prevented H2O2-induced cellular viability in HLE-B3 cells. Viability of HLE-B3 cells as assessed using CCK8 assay. HLE-B3 cells were treated with ML221 (10 µmol) for 1 h, preincubated with apelin-13 (0.1 µM) for 24 h, and subsequently treated with H2O2 (200 µM) for 24 h in this experiment. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Journal: BMC ophthalmology

Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

doi: 10.1186/s12886-024-03746-6

Figure Lengend Snippet: Fig. 3 Apelin-13 prevented H2O2-induced cellular viability in HLE-B3 cells. Viability of HLE-B3 cells as assessed using CCK8 assay. HLE-B3 cells were treated with ML221 (10 µmol) for 1 h, preincubated with apelin-13 (0.1 µM) for 24 h, and subsequently treated with H2O2 (200 µM) for 24 h in this experiment. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

Techniques: CCK-8 Assay, Comparison, Control

Fig. 4 Apelin-13 prevented H2O2-induced cellular apoptosis in HLE-B3 cells. Based on the experimental statistics, apelin-13 substantially decreased the apoptosis rate of HLE-B3 cells through APJ. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Journal: BMC ophthalmology

Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

doi: 10.1186/s12886-024-03746-6

Figure Lengend Snippet: Fig. 4 Apelin-13 prevented H2O2-induced cellular apoptosis in HLE-B3 cells. Based on the experimental statistics, apelin-13 substantially decreased the apoptosis rate of HLE-B3 cells through APJ. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3)

Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

Techniques: Comparison, Control

Fig. 5 Effect of apelin‑13 on mitochondrial apoptotic pathway in H2O2-induced HLE-B3 cells via APJ. (a, d) The fluorescence microscopy findings (×100) on mitochondrial membrane potential (MMP) in each group obtained using the fluorescence probe JC-1. (b, c) The expression of mitochondrial apop totic pathway-related proteins (Bax, Caspase-3, and Bcl-2) detected by western blotting. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3). The full-length blots/gels are presented in Supplementary Figs. 8–11

Journal: BMC ophthalmology

Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

doi: 10.1186/s12886-024-03746-6

Figure Lengend Snippet: Fig. 5 Effect of apelin‑13 on mitochondrial apoptotic pathway in H2O2-induced HLE-B3 cells via APJ. (a, d) The fluorescence microscopy findings (×100) on mitochondrial membrane potential (MMP) in each group obtained using the fluorescence probe JC-1. (b, c) The expression of mitochondrial apop totic pathway-related proteins (Bax, Caspase-3, and Bcl-2) detected by western blotting. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to comparison with the control group. Data are shown as mean ± SD (n = 3). The full-length blots/gels are presented in Supplementary Figs. 8–11

Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

Techniques: Fluorescence, Microscopy, Membrane, Expressing, Western Blot, Comparison, Control

Fig. 7 Apelin-13 regulated the intracellular redox state in H2O2 condi tions. (a) GSH/GSSG of HLE-B3 cells was detected using the GSH/GSSG kit. (b) NADPH/NADP + of HLE-B3 cells was detected using the NADPH/ NADP + kit. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to a comparison with the control group. Data are shown as mean ± SD (n = 3)

Journal: BMC ophthalmology

Article Title: Protective effect of apelin-13 in lens epithelial cells via inhibiting oxidative stress-induced apoptosis.

doi: 10.1186/s12886-024-03746-6

Figure Lengend Snippet: Fig. 7 Apelin-13 regulated the intracellular redox state in H2O2 condi tions. (a) GSH/GSSG of HLE-B3 cells was detected using the GSH/GSSG kit. (b) NADPH/NADP + of HLE-B3 cells was detected using the NADPH/ NADP + kit. Representative images of three replicated experiments were presented. The significance marked at the top of the columns refers to a comparison with the control group. Data are shown as mean ± SD (n = 3)

Article Snippet: HLEC-B3 cells culture and drug treatment The human lens epithelial B3 (HLE-B3) cell line (CRL11421; ATCC, Manassas, Virginia, USA) was used in this experiment.

Techniques: Comparison, Control

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